<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Stewart, Lucy C</style></author><author><style face="normal" font="default" size="100%">Llewellyn, James G</style></author><author><style face="normal" font="default" size="100%">Butterfield, David A</style></author><author><style face="normal" font="default" size="100%">Lilley, Marvin D</style></author><author><style face="normal" font="default" size="100%">Holden, James F</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Hydrogen and thiosulfate limits for growth of a thermophilic, autotrophic Desulfurobacterium species from a deep-sea hydrothermal vent.</style></title><secondary-title><style face="normal" font="default" size="100%">Environ Microbiol Rep</style></secondary-title><alt-title><style face="normal" font="default" size="100%">Environ Microbiol Rep</style></alt-title></titles><dates><year><style  face="normal" font="default" size="100%">2016</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2016 Apr</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">8</style></volume><pages><style face="normal" font="default" size="100%">196-200</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Hydrothermal fluids (341°C and 19°C) were collected &lt; 1 m apart from a black smoker chimney and a tubeworm mound on the Boardwalk edifice at the Endeavour Segment in the northeastern Pacific Ocean to study anaerobic microbial growth in hydrothermal mineral deposits. Geochemical modelling of mixed vent fluid and seawater suggests the mixture was anoxic above 55°C and that low H2 concentrations (79 μmol kg(-1) in end-member hydrothermal fluid) limit anaerobic hydrogenotrophic growth above this temperature. A thermophilic, hydrogenotrophic sulfur reducer, Desulfurobacterium strain HR11, was isolated from the 19°C fluid raising questions about its H2 -dependent growth kinetics. Strain HR11 grew at 40-77°C (Topt 72-75°C), pH 5-8.5 (pHopt 6-7) and 1-5% (wt vol(-1) ) NaCl (NaClopt 3-4%). The highest growth rates occurred when S2 O3 (2-) and S° were reduced to H2 S. Modest growth occurred by NO3 (-) reduction. Monod constants for its growth were Ks of 30 μM for H2 and Ks of 20 μM for S2 O3 (2-) with a μmax of 2.0 h(-1) . The minimum H2 and S2 O3 (2-) concentrations for growth were 3 μM and 5 μM respectively. Possible sources of S2 O3 (2-) and S° are from abiotic dissolved sulfide and pyrite oxidation by O2 .&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">2</style></issue><custom1><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/26696328?dopt=Abstract</style></custom1></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Kim, You-Tae</style></author><author><style face="normal" font="default" size="100%">Jung, Jong-Hyun</style></author><author><style face="normal" font="default" size="100%">Stewart, Lucy C</style></author><author><style face="normal" font="default" size="100%">Kwon, Soon-Wo</style></author><author><style face="normal" font="default" size="100%">Holden, James F</style></author><author><style face="normal" font="default" size="100%">Park, Cheon-Seok</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Complete genome sequence of the hyperthermophilic methanogen Methanocaldococcus bathoardescens JH146(T) isolated from the basalt subseafloor.</style></title><secondary-title><style face="normal" font="default" size="100%">Mar Genomics</style></secondary-title><alt-title><style face="normal" font="default" size="100%">Mar Genomics</style></alt-title></titles><dates><year><style  face="normal" font="default" size="100%">2015</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2015 Dec</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">24 Pt 3</style></volume><pages><style face="normal" font="default" size="100%">229-30</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Methanocaldococcus bathoardescens JH146(T) is a hyperthermophilic and obligate hydrogenotrophic methanogen isolated from low-temperature (26 °C) hydrothermal vent fluid at Axial Seamount in the northeastern Pacific Ocean. It is most closely related to the N2-fixing methanogen Methanocaldococcus sp. FS406-22; however, they differ in that JH146 cannot fix N2 or reductively assimilate nitrate. In this study, we present the complete genome sequence of strain JH146(T) (1,607,556 bp) with its 1635 protein coding genes, and 41 RNA genes. Our analysis focuses on its methane production via the acetyl-CoA pathway and its deleted gene clusters related to nitrogen assimilation. This study extends our understanding of methanogenesis at high temperatures and the impact of these organisms on the biogeochemistry of subseafloor hydrothermal environments and the deep sea.&lt;/p&gt;</style></abstract><custom1><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/26094864?dopt=Abstract</style></custom1></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Wang, David T</style></author><author><style face="normal" font="default" size="100%">Gruen, Danielle S</style></author><author><style face="normal" font="default" size="100%">Lollar, Barbara Sherwood</style></author><author><style face="normal" font="default" size="100%">Hinrichs, Kai-Uwe</style></author><author><style face="normal" font="default" size="100%">Stewart, Lucy C</style></author><author><style face="normal" font="default" size="100%">Holden, James F</style></author><author><style face="normal" font="default" size="100%">Hristov, Alexander N</style></author><author><style face="normal" font="default" size="100%">Pohlman, John W</style></author><author><style face="normal" font="default" size="100%">Morrill, Penny L</style></author><author><style face="normal" font="default" size="100%">Könneke, Martin</style></author><author><style face="normal" font="default" size="100%">Delwiche, Kyle B</style></author><author><style face="normal" font="default" size="100%">Reeves, Eoghan P</style></author><author><style face="normal" font="default" size="100%">Sutcliffe, Chelsea N</style></author><author><style face="normal" font="default" size="100%">Ritter, Daniel J</style></author><author><style face="normal" font="default" size="100%">Seewald, Jeffrey S</style></author><author><style face="normal" font="default" size="100%">McIntosh, Jennifer C</style></author><author><style face="normal" font="default" size="100%">Hemond, Harold F</style></author><author><style face="normal" font="default" size="100%">Kubo, Michael D</style></author><author><style face="normal" font="default" size="100%">Cardace, Dawn</style></author><author><style face="normal" font="default" size="100%">Hoehler, Tori M</style></author><author><style face="normal" font="default" size="100%">Ono, Shuhei</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Methane cycling. Nonequilibrium clumped isotope signals in microbial methane.</style></title><secondary-title><style face="normal" font="default" size="100%">Science</style></secondary-title><alt-title><style face="normal" font="default" size="100%">Science</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Animals</style></keyword><keyword><style  face="normal" font="default" size="100%">Carbon Cycle</style></keyword><keyword><style  face="normal" font="default" size="100%">Carbon Isotopes</style></keyword><keyword><style  face="normal" font="default" size="100%">Cattle</style></keyword><keyword><style  face="normal" font="default" size="100%">Groundwater</style></keyword><keyword><style  face="normal" font="default" size="100%">Hydrogen</style></keyword><keyword><style  face="normal" font="default" size="100%">Methane</style></keyword><keyword><style  face="normal" font="default" size="100%">Methanomicrobiales</style></keyword><keyword><style  face="normal" font="default" size="100%">Temperature</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2015</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2015 Apr 24</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">348</style></volume><pages><style face="normal" font="default" size="100%">428-31</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Methane is a key component in the global carbon cycle, with a wide range of anthropogenic and natural sources. Although isotopic compositions of methane have traditionally aided source identification, the abundance of its multiply substituted &quot;clumped&quot; isotopologues (for example, (13)CH3D) has recently emerged as a proxy for determining methane-formation temperatures. However, the effect of biological processes on methane's clumped isotopologue signature is poorly constrained. We show that methanogenesis proceeding at relatively high rates in cattle, surface environments, and laboratory cultures exerts kinetic control on (13)CH3D abundances and results in anomalously elevated formation-temperature estimates. We demonstrate quantitatively that H2 availability accounts for this effect. Clumped methane thermometry can therefore provide constraints on the generation of methane in diverse settings, including continental serpentinization sites and ancient, deep groundwaters.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">6233</style></issue><custom1><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/25745067?dopt=Abstract</style></custom1></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Stewart, Lucy C</style></author><author><style face="normal" font="default" size="100%">Jung, Jong-Hyun</style></author><author><style face="normal" font="default" size="100%">Kim, You-Tae</style></author><author><style face="normal" font="default" size="100%">Kwon, Soon-Wo</style></author><author><style face="normal" font="default" size="100%">Park, Cheon-Seok</style></author><author><style face="normal" font="default" size="100%">Holden, James F</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Methanocaldococcus bathoardescens sp. nov., a hyperthermophilic methanogen isolated from a volcanically active deep-sea hydrothermal vent.</style></title><secondary-title><style face="normal" font="default" size="100%">Int J Syst Evol Microbiol</style></secondary-title><alt-title><style face="normal" font="default" size="100%">Int. J. Syst. Evol. Microbiol.</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">DNA, Bacterial</style></keyword><keyword><style  face="normal" font="default" size="100%">Hydrothermal Vents</style></keyword><keyword><style  face="normal" font="default" size="100%">Methanocaldococcus</style></keyword><keyword><style  face="normal" font="default" size="100%">Molecular Sequence Data</style></keyword><keyword><style  face="normal" font="default" size="100%">Pacific Ocean</style></keyword><keyword><style  face="normal" font="default" size="100%">Phylogeny</style></keyword><keyword><style  face="normal" font="default" size="100%">RNA, Ribosomal, 16S</style></keyword><keyword><style  face="normal" font="default" size="100%">Seawater</style></keyword><keyword><style  face="normal" font="default" size="100%">Sequence Analysis, DNA</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2015</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2015 Apr</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">65</style></volume><pages><style face="normal" font="default" size="100%">1280-3</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;A hyperthermophilic methanogen, strain JH146(T), was isolated from 26 °C hydrothermal vent fluid emanating from a crack in basaltic rock at Marker 113 vent, Axial Seamount in the northeastern Pacific Ocean. It was identified as an obligate anaerobe that uses only H2 and CO2 for growth. Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain is more than 97% similar to other species of the genus Methanocaldococcus . Therefore, overall genome relatedness index analyses were performed to establish that strain JH146(T) represents a novel species. For each analysis, strain JH146(T) was most similar to Methanocaldococcus sp. FS406-22, which can fix N2 and also comes from Marker 113 vent. However, strain JH146(T) differs from strain FS406-22 in that it cannot fix N2. The average nucleotide identity score for strain JH146(T) was 87%, the genome-to-genome direct comparison score was 33-55% and the species identification score was 93%. For each analysis, strain JH146(T) was below the species delineation cut-off. Full-genome gene synteny analysis showed that strain JH146(T) and strain FS406-22 have 97% genome synteny, but strain JH146(T) was missing the operons necessary for N2 fixation and assimilatory nitrate reduction that are present in strain FS406-22. Based on its whole genome sequence, strain JH146(T) is suggested to represent a novel species of the genus Methanocaldococcus for which the name Methanocaldococcus bathoardescens is proposed. The type strain is JH146(T) ( = DSM 27223(T) = KACC 18232(T)).&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">Pt 4</style></issue><custom1><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/25634941?dopt=Abstract</style></custom1></record></records></xml>