<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Siegrist, M Sloan</style></author><author><style face="normal" font="default" size="100%">Whiteside, Sarah</style></author><author><style face="normal" font="default" size="100%">Jewett, John C</style></author><author><style face="normal" font="default" size="100%">Aditham, Arjun</style></author><author><style face="normal" font="default" size="100%">Cava, Felipe</style></author><author><style face="normal" font="default" size="100%">Bertozzi, Carolyn R</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">(D)-Amino acid chemical reporters reveal peptidoglycan dynamics of an intracellular pathogen.</style></title><secondary-title><style face="normal" font="default" size="100%">ACS Chem Biol</style></secondary-title><alt-title><style face="normal" font="default" size="100%">ACS Chem. Biol.</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Alanine</style></keyword><keyword><style  face="normal" font="default" size="100%">Click Chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Listeria monocytogenes</style></keyword><keyword><style  face="normal" font="default" size="100%">Molecular Conformation</style></keyword><keyword><style  face="normal" font="default" size="100%">Molecular Dynamics Simulation</style></keyword><keyword><style  face="normal" font="default" size="100%">Peptidoglycan</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2013</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2013 Mar 15</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">8</style></volume><pages><style face="normal" font="default" size="100%">500-5</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Peptidoglycan (PG) is an essential component of the bacterial cell wall. Although experiments with organisms in vitro have yielded a wealth of information on PG synthesis and maturation, it is unclear how these studies translate to bacteria replicating within host cells. We report a chemical approach for probing PG in vivo via metabolic labeling and bioorthogonal chemistry. A wide variety of bacterial species incorporated azide and alkyne-functionalized d-alanine into their cell walls, which we visualized by covalent reaction with click chemistry probes. The d-alanine analogues were specifically incorporated into nascent PG of the intracellular pathogen Listeria monocytogenes both in vitro and during macrophage infection. Metabolic incorporation of d-alanine derivatives and click chemistry detection constitute a facile, modular platform that facilitates unprecedented spatial and temporal resolution of PG dynamics in vivo.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><custom1><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/23240806?dopt=Abstract</style></custom1></record></records></xml>