@article {3109,
	title = {Enhancing syntrophic metabolism in up-flow anaerobic sludge blanket reactors with conductive carbon materials.},
	journal = {Bioresour Technol},
	volume = {191},
	year = {2015},
	month = {2015 Sep},
	pages = {140-5},
	abstract = {<p>Syntrophic metabolism of alcohols and fatty acids is a critical step in anaerobic digestion, which if enhanced can better stabilize the process and enable shorter retention times. Direct interspecies electron transfer (DIET) has recently been recognized as an alternative route to hydrogen interspecies transfer as a mechanism for interspecies syntrophic electron exchange. Therefore, the possibility of accelerating syntrophic metabolism of ethanol in up-flow anaerobic sludge blanket (UASB) reactors by incorporating conductive materials in reactor design was investigated. Graphite, biochar, and carbon cloth all immediately enhanced methane production and COD removal. As the hydraulic retention time was decreased the increased effectiveness of treatment in reactors with conductive materials increased versus the control reactor. When these conductive materials were removed from the reactors rates of syntrophic metabolism declined to rates comparable to the control reactor. These results suggest that incorporating conductive materials in the design of UASB reactors may enhance digester effectiveness.</p>},
	keywords = {Anaerobiosis, Bioreactors, Carbon, Charcoal, Electron Transport, Graphite, Hydrogen, Methane, Sewage, Waste Disposal, Fluid},
	issn = {1873-2976},
	doi = {10.1016/j.biortech.2015.05.007},
	author = {Zhao, Zhiqiang and Zhang, Yaobin and Woodard, T L and Nevin, K P and Lovley, D R}
}
@article {557,
	title = {Geobacter sulfurreducens can grow with oxygen as a terminal electron acceptor.},
	journal = {Appl Environ Microbiol},
	volume = {70},
	year = {2004},
	month = {2004 Apr},
	pages = {2525-8},
	abstract = {Geobacter sulfurreducens, previously classified as a strict anaerobe, tolerated exposure to atmospheric oxygen for at least 24 h and grew with oxygen as the sole electron acceptor at concentrations of 10\% or less in the headspace. These results help explain how Geobacter species may survive in oxic subsurface environments, being poised to rapidly take advantage of the development of anoxic conditions.},
	keywords = {Aerobiosis, Anaerobiosis, Electron Transport, Environmental Microbiology, Geobacter, Oxygen},
	issn = {0099-2240},
	author = {Lin, W C and Coppi, M V and Lovley, D R}
}
@article {562,
	title = {Genome of Geobacter sulfurreducens: metal reduction in subsurface environments.},
	journal = {Science},
	volume = {302},
	year = {2003},
	month = {2003 Dec 12},
	pages = {1967-9},
	abstract = {The complete genome sequence of Geobacter sulfurreducens, a delta-proteobacterium, reveals unsuspected capabilities, including evidence of aerobic metabolism, one-carbon and complex carbon metabolism, motility, and chemotactic behavior. These characteristics, coupled with the possession of many two-component sensors and many c-type cytochromes, reveal an ability to create alternative, redundant, electron transport networks and offer insights into the process of metal ion reduction in subsurface environments. As well as playing roles in the global cycling of metals and carbon, this organism clearly has the potential for use in bioremediation of radioactive metals and in the generation of electricity.},
	keywords = {Acetates, Acetyl Coenzyme A, Aerobiosis, Anaerobiosis, Bacterial Proteins, Carbon, Chemotaxis, Chromosomes, Bacterial, Cytochromes c, Electron Transport, Energy Metabolism, Genes, Bacterial, Genes, Regulator, Genome, Bacterial, Geobacter, Hydrogen, Metals, Movement, Open Reading Frames, Oxidation-Reduction, Phylogeny},
	issn = {1095-9203},
	doi = {10.1126/science.1088727},
	author = {Meth{\'e}, B A and Nelson, K E and Eisen, J A and Paulsen, I T and Nelson, W and Heidelberg, J F and Wu, D and Wu, M and Ward, N and Beanan, M J and Dodson, R J and Madupu, R and Brinkac, L M and Daugherty, S C and DeBoy, R T and Durkin, A S and Gwinn, M and Kolonay, J F and Sullivan, S A and Haft, D H and Selengut, J and Davidsen, T M and Zafar, N and White, O and Tran, B and Romero, C and Forberger, H A and Weidman, J and Khouri, H and Feldblyum, T V and Utterback, T R and Van Aken, S E and Lovley, D R and Fraser, C M}
}
@article {603,
	title = {Acetate oxidation coupled to Fe(iii) reduction in hyperthermophilic microorganisms.},
	journal = {Appl Environ Microbiol},
	volume = {67},
	year = {2001},
	month = {2001 Mar},
	pages = {1363-5},
	abstract = {No hyperthermophilic microorganisms have previously been shown to anaerobically oxidize acetate, the key extracellular intermediate in the anaerobic oxidation of organic matter. Here we report that two hyperthermophiles, Ferroglobus placidus and "Geoglobus ahangari," grow at 85 degrees C by oxidizing acetate to carbon dioxide, with Fe(III) serving as the electron acceptor. These results demonstrate that acetate could potentially be metabolized within the hot microbial ecosystems in which hyperthermophiles predominate, rather than diffusing to cooler environments prior to degradation as has been previously proposed.},
	keywords = {Acetates, Anaerobiosis, Archaea, Ferric Compounds, Hot Temperature, Oxidation-Reduction},
	issn = {0099-2240},
	doi = {10.1128/AEM.67.3.1363-1365.2001},
	author = {Tor, J M and Kashefi, K and Lovley, D R}
}
@article {598,
	title = {Anaerobic degradation of aromatic compounds coupled to Fe(III) reduction by Ferroglobus placidus.},
	journal = {Environ Microbiol},
	volume = {3},
	year = {2001},
	month = {2001 Apr},
	pages = {281-7},
	abstract = {Aromatic compounds are an important component of the organic matter in some of the anaerobic environments that hyperthermophilic microorganisms inhabit, but the potential for hyperthermophilic microorganisms to metabolize aromatic compounds has not been described previously. In this study, aromatic metabolism was investigated in the hyperthermophile Ferroglobus placidus. F. placidus grew at 85 degrees C in anaerobic medium with a variety of aromatic compounds as the sole electron donor and poorly crystalline Fe(III) oxide as the electron acceptor. Growth coincided with Fe(III) reduction. Aromatic compounds supporting growth included benzoate, phenol, 4-hydroxybenzoate, benzaldehyde, p-hydroxybenzaldehyde and t-cinnamic acid (3-phenyl-2-propenoic acid). These aromatic compounds did not support growth when nitrate was provided as the electron acceptor, even though nitrate supports the growth of this organism with Fe(II) or H2 as the electron donor. The stoichiometry of benzoate and phenol uptake and Fe(III) reduction indicated that F. placidus completely oxidized these aromatic compounds to carbon dioxide, with Fe(III) serving as the sole electron acceptor. This is the first example of an Archaea that can anaerobically oxidize an aromatic compound. These results also demonstrate for the first time that hyperthermophilic microorganisms can anaerobically oxidize aromatic compounds and suggest that hyperthermophiles may metabolize aromatic compounds in hot environments such as the deep hot subsurface and in marine and terrestrial hydrothermal zones in which Fe(III) is available as an electron acceptor.},
	keywords = {Anaerobiosis, Archaea, Electron Transport, Ferric Compounds, Hot Temperature, Hydrocarbons, Aromatic, Oxidation-Reduction},
	issn = {1462-2912},
	author = {Tor, J M and Lovley, D R}
}
@article {599,
	title = {Anaerobic degradation of methyl tert-butyl ether (MTBE) and tert-butyl alcohol (TBA).},
	journal = {Environ Sci Technol},
	volume = {35},
	year = {2001},
	month = {2001 May 1},
	pages = {1785-90},
	abstract = {The potential for anaerobic degradation of methyl tert-butyl ether (MTBE) and tert-butyl alcohol (TBA) was investigated in laboratory incubations of sediments from a petroleum-contaminated aquifer and in aquatic sediments. The addition of humic substances (HS) stimulated the anaerobic degradation of MTBE in aquifer sediments in which Fe(III) was available as an electron acceptor. This is attributed to the fact that HS and other extracellular quinones can stimulate the activity of Fe(III)-reducing microorganisms by acting as an electron shuttle between Fe(III)-reducing microorganisms and insoluble Fe(III) oxides. MTBE was not degraded in aquifer sediments without Fe(III) and HS. [14C]-MTBE added to aquatic sediments adapted for anaerobic MTBE degradation was converted to 14CO2 in the presence or absence of HS or the HS analog, anthraquione-2,6-disulfonate. Unamended aquatic sediments produced 14CH4 as well as 14CO2 from [14C]-MTBE. The aquatic sediments also rapidly consumed TBA under anaerobic conditions and converted [14C]-TBA to 14CH4 and 14CO2. An adaptation period of ca. 250-300 days was required prior to the most rapid anaerobic MTBE degradation in both sediment types, whereas TBA was metabolized in the aquatic sediments without a lag. These results demonstrate that, under the appropriate conditions, MTBE and TBA can be degraded in the absence of oxygen. This suggests that it may be possible to design strategies for the anaerobic remediation of MTBE in petroleum-contaminated subsurface environments.},
	keywords = {Anaerobiosis, Biodegradation, Environmental, Carbon Dioxide, Geologic Sediments, Methane, Methyl Ethers, tert-Butyl Alcohol, Water Pollutants, Chemical},
	issn = {0013-936X},
	author = {Finneran, K T and Lovley, D R}
}
@article {612,
	title = {Hexadecane decay by methanogenesis.},
	journal = {Nature},
	volume = {404},
	year = {2000},
	month = {2000 Apr 13},
	pages = {722-3},
	keywords = {Alkanes, Anaerobiosis, Bacteria, Biodegradation, Environmental, Geologic Sediments, Methane, Petroleum},
	issn = {0028-0836},
	doi = {10.1038/35008145},
	author = {Anderson, R T and Lovley, D R}
}
@article {608,
	title = {Novel forms of anaerobic respiration of environmental relevance.},
	journal = {Curr Opin Microbiol},
	volume = {3},
	year = {2000},
	month = {2000 Jun},
	pages = {252-6},
	abstract = {Novel forms of anaerobic respiration continue to be discovered. Many of these are environmentally significant as they have important impacts on the fate of organic carbon and the cycling of many inorganic compounds. Furthermore, anaerobic respiration is becoming increasing recognized as a strategy for the remediation of organic and metal contaminants in the subsurface.},
	keywords = {Anaerobiosis, Bacteria, Anaerobic, Environment, Industrial Microbiology, Industrial Waste},
	issn = {1369-5274},
	author = {Lovley, D R and Coates, J D}
}
@article {605,
	title = {Humics as an electron donor for anaerobic respiration.},
	journal = {Environ Microbiol},
	volume = {1},
	year = {1999},
	month = {1999 Feb},
	pages = {89-98},
	abstract = {The possibility that microorganisms might use reduced humic substances (humics) as an electron donor for the reduction of electron acceptors with a more positive redox potential was investigated. All of the Fe(III)- and humics-reducing microorganisms evaluated were capable of oxidizing reduced humics and/or the reduced humics analogue anthrahydroquinone-2,6,-disulphonate (AHODS), with nitrate and/or fumarate as the electron acceptor. These included Geobacter metallireducens, Geobacter sulphurreducens, Geothrix fermentans, Shewanella alga, Wolinella succinogenes and {\textquoteright}S. barnesii{\textquoteright}. Several of the humics-oxidizing microorganisms grew in medium with AHQDS as the sole electron donor and fumarate as the electron acceptor. Even though it does not reduce Fe(III) or humics, Paracoccus denitrificans could use AHQDS and reduced humics as electron donors for denitrification. However, another denitrifier, Pseudomonas denitrificans, could not. AHODS could also serve as an electron donor for selenate and arsenate reduction by W. succinogenes. Electron spin resonance studies demonstrated that humics oxidation was associated with the oxidation of hydroquinone moieties in the humics. Studies with G. metallireducens and W. succinogenes demonstrated that the anthraquinone-2,6-disulphonate (AQDS)/AHQDS redox couple mediated an interspecies electron transfer between the two organisms. These results suggest that, as microbially reduced humics enter less reduced zones of soils and sediments, the reduced humics may serve as electron donors for microbial reduction of several environmentally significant electron acceptors.},
	keywords = {Aerobiosis, Anaerobiosis, Anthraquinones, Arsenates, Bacteria, Electron Transport, Fumarates, Humic Substances, Selenium Compounds, Tumor Cells, Cultured},
	issn = {1462-2912},
	author = {Lovley, D R and Fraga, J L and Coates, J D and Blunt-Harris, E L}
}
@article {619,
	title = {Microbial communities associated with anaerobic benzene degradation in a petroleum-contaminated aquifer.},
	journal = {Appl Environ Microbiol},
	volume = {65},
	year = {1999},
	month = {1999 Jul},
	pages = {3056-63},
	abstract = {Microbial community composition associated with benzene oxidation under in situ Fe(III)-reducing conditions in a petroleum-contaminated aquifer located in Bemidji, Minn., was investigated. Community structure associated with benzene degradation was compared to sediment communities that did not anaerobically oxidize benzene which were obtained from two adjacent Fe(III)-reducing sites and from methanogenic and uncontaminated zones. Denaturing gradient gel electrophoresis of 16S rDNA sequences amplified with bacterial or Geobacteraceae-specific primers indicated significant differences in the composition of the microbial communities at the different sites. Most notable was a selective enrichment of microorganisms in the Geobacter cluster seen in the benzene-degrading sediments. This finding was in accordance with phospholipid fatty acid analysis and most-probable-number-PCR enumeration, which indicated that members of the family Geobacteraceae were more numerous in these sediments. A benzene-oxidizing Fe(III)-reducing enrichment culture was established from benzene-degrading sediments and contained an organism closely related to the uncultivated Geobacter spp. This genus contains the only known organisms that can oxidize aromatic compounds with the reduction of Fe(III). Sequences closely related to the Fe(III) reducer Geothrix fermentans and the aerobe Variovorax paradoxus were also amplified from the benzene-degrading enrichment and were present in the benzene-degrading sediments. However, neither G. fermentans nor V. paradoxus is known to oxidize aromatic compounds with the reduction of Fe(III), and there was no apparent enrichment of these organisms in the benzene-degrading sediments. These results suggest that Geobacter spp. play an important role in the anaerobic oxidation of benzene in the Bemidji aquifer and that molecular community analysis may be a powerful tool for predicting a site{\textquoteright}s capacity for anaerobic benzene degradation.},
	keywords = {Anaerobiosis, Benzene, Biodegradation, Environmental, Culture Media, DNA, Bacterial, DNA, Ribosomal, Fresh Water, Geologic Sediments, Gram-Negative Anaerobic Bacteria, Molecular Sequence Data, Oxidation-Reduction, Petroleum, Phylogeny, Polymerase Chain Reaction, RNA, Ribosomal, 16S, Sequence Analysis, DNA, Water Pollutants},
	issn = {0099-2240},
	author = {Rooney-Varga, J N and Anderson, R T and Fraga, J L and Ringelberg, D and Lovley, D R}
}
@article {626,
	title = {Anaerobic benzene degradation in petroleum-contaminated aquifer sediments after inoculation with a benzene-oxidizing enrichment.},
	journal = {Appl Environ Microbiol},
	volume = {64},
	year = {1998},
	month = {1998 Feb},
	pages = {775-8},
	abstract = {Sediments from the sulfate-reduction zone of a petroleum-contaminated aquifer, in which benzene persisted, were inoculated with a benzene-oxidizing, sulfate-reducing enrichment from aquatic sediments. Benzene was degraded, with apparent growth of the benzene-degrading population over time. These results suggest that the lack of benzene degradation in the sulfate-reduction zones of some aquifers may result from the failure of the appropriate benzene-degrading sulfate reducers to colonize the aquifers rather than from environmental conditions that are adverse for anaerobic benzene degradation.},
	keywords = {Anaerobiosis, Benzene, Biodegradation, Environmental, Fresh Water, Geologic Sediments, Iron, Oxidation-Reduction, Petroleum, Sulfates},
	issn = {0099-2240},
	author = {Weiner, JM and Lovley, D R}
}
@article {622,
	title = {Humic acids as electron acceptors for anaerobic microbial oxidation of vinyl chloride and dichloroethene.},
	journal = {Appl Environ Microbiol},
	volume = {64},
	year = {1998},
	month = {1998 Aug},
	pages = {3102-5},
	abstract = {Anaerobic oxidation of [1,2-14C]vinyl chloride and [1, 2-14C]dichloroethene to 14CO2 under humic acid-reducing conditions was demonstrated. The results indicate that waterborne contaminants can be oxidized by using humic acid compounds as electron acceptors and suggest that natural aquatic systems have a much larger capacity for contaminant oxidation than previously thought.},
	keywords = {Aerobiosis, Anaerobiosis, Bacteria, Biodegradation, Environmental, Geologic Sediments, Humic Substances, Oxidation-Reduction, Vinyl Chloride, Water Microbiology, Water Pollutants},
	issn = {0099-2240},
	author = {Bradley, P M and Chapelle, F H and Lovley, D R}
}
@article {628,
	title = {Anaerobic degradation of polycyclic aromatic hydrocarbons and alkanes in petroleum-contaminated marine harbor sediments.},
	journal = {Appl Environ Microbiol},
	volume = {63},
	year = {1997},
	month = {1997 Sep},
	pages = {3589-93},
	abstract = {Although polycyclic aromatic hydrocarbons (PAHs) have usually been found to persist under strict anaerobic conditions, in a previous study an unusual site was found in San Diego Bay in which two PAHs, naphthalene and phenanthrene, were oxidized to carbon dioxide under sulfate-reducing conditions. Further investigations with these sediments revealed that methylnaphthalene, fluorene, and fluoranthene were also anaerobically oxidized to carbon dioxide in these sediments, while pyrene and benzo[a]pyrene were not. Studies with naphthalene indicated that PAH oxidation was sulfate dependent. Incubating the sediments with additional naphthalene for 1 month resulted in a significant increase in the oxidation of [14C]naphthalene. In sediments from a less heavily contaminated site in San diego Bay where PAHs were not readily degraded, naphthalene degradation could be stimulated through inoculation with active PAH-degrading sediments from the most heavily contaminated site. Sediments from the less heavily contaminated site that had been adapted for rapid anaerobic degradation of high concentrations of benzene did not oxidize naphthalene, suggesting that the benzene- and naphthalene-degrading populations were different. When fuels containing complex mixtures of alkanes were added to sediments from the two sites, there was significant degradation in the alkanes. [14C]hexadecane was also anaerobically oxidized to 14CO2 in these sediments. Molybdate, a specific inhibitor of sulfate reduction, inhibited hexadecane oxidation. These results demonstrate that a wide variety of hydrocarbon contaminants can be degraded under sulfate-reducing conditions in hydrocarbon-contaminated sediments, and they suggest that it may be possible to use sulfate reduction rather than aerobic respiration as a treatment strategy for hydrocarbon-contaminated dredged sediments.},
	keywords = {Alkanes, Anaerobiosis, Biodegradation, Environmental, California, Naphthalenes, Oxidation-Reduction, Petroleum, Polycyclic Hydrocarbons, Aromatic, Sulfates, Water Microbiology, Water Pollutants, Chemical},
	issn = {0099-2240},
	author = {Coates, J D and Woodward, J and Allen, J and Philp, P and Lovley, D R}
}
