@article {3070,
	title = {An  Chassis for Production of Electrically Conductive Protein Nanowires.},
	journal = {ACS Synth Biol},
	volume = {9},
	year = {2020},
	month = {2020 Mar 20},
	pages = {647-654},
	abstract = {<p> pilin-based electrically conductive protein nanowires (e-PNs) are a revolutionary electronic material. They offer novel options for electronic sensing applications and have the remarkable ability to harvest electrical energy from atmospheric humidity. However, technical constraints limit mass cultivation and genetic manipulation of . Therefore, we designed a strain of  to express e-PNs by introducing a plasmid that contained an inducible operon with  genes for type IV pili biogenesis machinery and a synthetic gene designed to yield a peptide monomer that could be assembled into e-PNs. The e-PNs expressed in  and harvested with a simple filtration method had the same diameter (3 nm) and conductance as e-PNs expressed in . These results, coupled with the robustness of  for mass cultivation and the extensive  toolbox for genetic manipulation, greatly expand the opportunities for large-scale fabrication of novel e-PNs.</p>},
	keywords = {Electric Conductivity, Escherichia coli, Fimbriae Proteins, Fimbriae, Bacterial, Geobacter, Graphite, Microorganisms, Genetically-Modified, Microscopy, Atomic Force, Nanowires, Operon, Protein Engineering},
	issn = {2161-5063},
	doi = {10.1021/acssynbio.9b00506},
	author = {Ueki, Toshiyuki and Walker, David J F and Woodard, Trevor L and Nevin, Kelly P and Nonnenmann, Stephen S and Lovley, Derek R}
}
