@article {401,
	title = {Measuring beta-galactosidase activity in bacteria: cell growth, permeabilization, and enzyme assays in 96-well arrays.},
	journal = {Biochem Biophys Res Commun},
	volume = {290},
	year = {2002},
	month = {2002 Jan 11},
	pages = {397-402},
	abstract = {We describe a high-throughput procedure for measuring beta-galactosidase activity in bacteria. This procedure is unique because all manipulations, including bacterial growth and cell permeabilization, are performed in a 96-well format. Cells are permeabilized by chloroform/SDS treatment directly in the 96-well blocks and then transferred to 96-well microplates for standard colorimetric assay of beta-galactosidase activity as described by Miller [J. H. Miller (1972) Experiments in Molecular Genetics, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY]. Absorbance data are collected with a microplate reader and analyzed using a Microsoft Excel spreadsheet. The beta-galactosidase specific activity values obtained with the high-throughput procedure are identical to those obtained by the traditional single-tube method of Miller. Thus, values obtained with this procedure may be expressed as Miller units and compared directly to Miller units reported in the literature. The 96-well format for permeabilization and assay of enzyme specific activity together with the use of 12-channel and repeater pipettors enables efficient processing of hundreds of samples in an 8-h day.},
	keywords = {Bacteria, beta-Galactosidase, Biochemistry, Cell Division, Chloroform, Escherichia coli, Polypropylenes, Sodium Dodecyl Sulfate, Software, Spectrophotometry, Time Factors},
	issn = {0006-291X},
	doi = {10.1006/bbrc.2001.6152},
	author = {Griffith, Kevin L and Wolf, Richard E}
}
