@article {1565,
	title = {Guided cobalamin biosynthesis supports Dehalococcoides mccartyi reductive dechlorination activity.},
	journal = {Philos Trans R Soc Lond B Biol Sci},
	volume = {368},
	year = {2013},
	month = {2013 Apr 19},
	pages = {20120320},
	abstract = {<p>Dehalococcoides mccartyi strains are corrinoid-auxotrophic Bacteria and axenic cultures that require vitamin B12 (CN-Cbl) to conserve energy via organohalide respiration. Cultures of D. mccartyi strains BAV1, GT and FL2 grown with limiting amounts of 1 {\textmu}g l(-1) CN-Cbl quickly depleted CN-Cbl, and reductive dechlorination of polychlorinated ethenes was incomplete leading to vinyl chloride (VC) accumulation. In contrast, the same cultures amended with 25 {\textmu}g l(-1) CN-Cbl exhibited up to 2.3-fold higher dechlorination rates, 2.8-9.1-fold increased growth yields, and completely consumed growth-supporting chlorinated ethenes. To explore whether known cobamide-producing microbes supply Dehalococcoides with the required corrinoid cofactor, co-culture experiments were performed with the methanogen Methanosarcina barkeri strain Fusaro and two acetogens, Sporomusa ovata and Sporomusa sp. strain KB-1, as Dehalococcoides partner populations. During growth with H2/CO2, M. barkeri axenic cultures produced 4.2 {\textpm} 0.1 {\textmu}g l(-1) extracellular cobamide (factor III), whereas the Sporomusa cultures produced phenolyl- and p-cresolyl-cobamides. Neither factor III nor the phenolic cobamides supported Dehalococcoides reductive dechlorination activity suggesting that M. barkeri and the Sporomusa sp. cannot fulfil Dehalococcoides{\textquoteright} nutritional requirements. Dehalococcoides dechlorination activity and growth occurred in M. barkeri and Sporomusa sp. co-cultures amended with 10 {\textmu}M 5{\textquoteright},6{\textquoteright}-dimethylbenzimidazole (DMB), indicating that a cobalamin is a preferred corrinoid cofactor of strains BAV1, GT and FL2 when grown with chlorinated ethenes as electron acceptors. Even though the methanogen and acetogen populations tested did not produce cobalamin, the addition of DMB enabled guided biosynthesis and generated a cobalamin that supported Dehalococcoides{\textquoteright} activity and growth. Guided cobalamin biosynthesis may offer opportunities to sustain and enhance Dehalococcoides activity in contaminated subsurface environments.</p>},
	keywords = {Bacteriological Techniques, Benzimidazoles, Biodegradation, Environmental, Chloroflexi, Chromatography, High Pressure Liquid, Coculture Techniques, Culture Media, Dichloroethylenes, Halogenation, Oxidation-Reduction, Trichloroethylene, Vitamin B 12},
	issn = {1471-2970},
	doi = {10.1098/rstb.2012.0320},
	author = {Yan, Jun and Im, Jeongdae and Yang, Yi and L{\"o}ffler, Frank E}
}
